› 7q7j

X-ray diffraction
2.69Å resolution

Room temperature structure of the Rhodobacter Sphaeroides Photosynthetic Reaction Center F(M197)H mutant at 75 MPa helium gas pressure in a sapphire capillary

Released:
DOI: 10.2210/pdb7q7j/pdb
PDB entry 7q7j coloured by chain, front view.
PDB entry 7q7j coloured by chain, side view.
PDB entry 7q7j coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Entry authors: Lieske J, Guenther S, Saouane S, Selikhanov GK, Gabdulkhakov AG, Meents A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 241 amino acids
Theoretical weight: 26.17 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 10-250; Coverage: 93%)
Gene name: puhA
Sequence domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.36 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 302 amino acids
Theoretical weight: 33.88 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-303; Coverage: 98%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein

Ligands and Environments

10 bound ligands:
Ligand LDA 9 x LDA
Ligand BPH 2 x BPH
Ligand BCL 4 x BCL
Ligand OLC 2 x OLC
Ligand UQ7 1 x UQ7
Ligand FE 1 x FE
Ligand PO4 2 x PO4
Ligand SPN 1 x SPN
Ligand CIT 1 x CIT
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P42212
Unit cell:
a: 102.3Å b: 102.3Å c: 237.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.231
Expression system: Cereibacter sphaeroides

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