7qbi

Electron Microscopy
3.46Å resolution

Structure of the GPCR dimer Ste2 in the active-like state bound to agonist

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Activation mechanism of the class D fungal GPCR dimer Ste2.
OpenAccess logo Nature 603 743-748 (2022)
PMID: 35296853
Related structures: EMD-13887

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-112815 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pheromone alpha factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 47.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Trichoplusia ni
UniProt:
  • Canonical: D6VTK4 (Residues: 1-431; Coverage: 100%)
Gene names: STE2, YFL026W
Sequence domains: Fungal pheromone mating factor STE2 GPCR
Mating factor alpha Chains: I, K
Molecule details ›
Chains: I, K
Length: 13 amino acids
Theoretical weight: 1.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P01149 (Residues: 153-165; Coverage: 9%)
Gene names: MF(ALPHA)1, MF-ALPHA-1, MFAL1, YPL187W
Sequence domains: Yeast mating factor alpha hormone

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.46Å
Relevant EMDB volumes: EMD-13887
Expression systems:
  • Trichoplusia ni
  • Not provided