› 7qe8

X-ray diffraction
2.9Å resolution

Human cationic trypsin (TRY1) complexed with serine protease inhibitor Kazal type 1 (SPINK1)

Released:
DOI: 10.2210/pdb7qe8/pdb
PDB entry 7qe8 coloured by chain, front view.
PDB entry 7qe8 coloured by chain, side view.
PDB entry 7qe8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and Biophysical Insights into SPINK1 Bound to Human Cationic Trypsin.
Nagel F, Palm GJ, Geist N, McDonnell TCR, Susemihl A, Girbardt B, Mayerle J, Lerch MM, Lammers M, Delcea M
OpenAccess logo Int J Mol Sci 23 (2022)
PMID: 35408828

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133771 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine protease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 24.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07477 (Residues: 24-247; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Serine protease inhibitor Kazal-type 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 56 amino acids
Theoretical weight: 6.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00995 (Residues: 24-79; Coverage: 100%)
Gene names: PSTI, SPINK1
Sequence domains: Kazal-type serine protease inhibitor domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 77.626Å b: 77.626Å c: 187.349Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.223 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Extracellular proteolysis in cancer: Proteases, substrates, and mechanisms in tumor progression and metastasis.
Radisky ES. (2024)
1 other article

2 mentions without citation

Structural and Biophysical Insights into SPINK1 Bound to Human Cationic Trypsin.
Nagel et al. (2022)
1 more