› 7qea

X-ray diffraction
2.28Å resolution

Crystal structure of fluorescein-di-Beta-D-glucuronide bound to a mutant of SN243 (D415A)

Released:
DOI: 10.2210/pdb7qea/pdb
PDB entry 7qea coloured by chain, front view.
PDB entry 7qea coloured by chain, side view.
PDB entry 7qea coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Functional metagenomic screening identifies an unexpected β-glucuronidase.
Neun S, Brear P, Campbell E, Tryfona T, El Omari K, Wagner A, Dupree P, Hyvönen M, Hollfelder F
Nat Chem Biol 18 1096-1103 (2022)
PMID: 35799064

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SN243 Chains: A, B
Molecule details ›
Chains: A, B
Length: 759 amino acids
Theoretical weight: 81.87 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand B9I 2 x B9I
Ligand ZN 38 x ZN
Ligand ACT 1 x ACT
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P1
Unit cell:
a: 63.268Å b: 81.565Å c: 93.393Å
α: 66.64° β: 89.38° γ: 89.57°
R-values:
R R work R free
0.201 0.198 0.252
Expression system: Escherichia coli

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Citations

1 review citation

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments.
Gantz et al. (2023)
5 other articles