› 7qgr

Electron Microscopy
5.7Å resolution

Structure of the SmrB-bound E. coli disome - collided 70S ribosome

Released:
DOI: 10.2210/pdb7qgr/pdb
PDB entry 7qgr coloured by chain, front view.
PDB entry 7qgr coloured by chain, side view.
PDB entry 7qgr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Ribosome collisions induce mRNA cleavage and ribosome rescue in bacteria.
Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR
Nature 603 503-508 (2022)
PMID: 35264790
Related structures: EMD-13958

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 59-mer (preferred)
PDBe Complex ID:
PDB-CPX-279458 (preferred)
Entry contents:
53 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (59 distinct):
Large ribosomal subunit protein uL2 Chain: P
Molecule details ›
Chain: P
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: Q
Molecule details ›
Chain: Q
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: R
Molecule details ›
Chain: R
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL5 Chain: S
Molecule details ›
Chain: S
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P62399 (Residues: 1-179; Coverage: 100%)
Gene names: JW3270, b3308, rplE
Sequence domains:
Large ribosomal subunit protein uL6 Chain: T
Molecule details ›
Chain: T
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG55 (Residues: 1-177; Coverage: 100%)
Gene names: JW3267, b3305, rplF
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein uL11 Chain: V
Molecule details ›
Chain: V
Length: 142 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7J7 (Residues: 1-142; Coverage: 100%)
Gene names: JW3946, b3983, relC, rplK
Sequence domains:
Large ribosomal subunit protein uL13 Chain: W
Molecule details ›
Chain: W
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: X
Molecule details ›
Chain: X
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY3 (Residues: 1-123; Coverage: 100%)
Gene names: JW3272, b3310, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: Y
Molecule details ›
Chain: Y
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02413 (Residues: 1-144; Coverage: 100%)
Gene names: JW3263, b3301, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: Z
Molecule details ›
Chain: Z
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)
Gene names: JW3275, b3313, rplP
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein bL17 Chain: a
Molecule details ›
Chain: a
Length: 127 amino acids
Theoretical weight: 14.34 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG44 (Residues: 1-127; Coverage: 100%)
Gene names: JW3256, b3294, rplQ
Sequence domains: Ribosomal protein L17
Large ribosomal subunit protein uL18 Chain: b
Molecule details ›
Chain: b
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0C018 (Residues: 1-117; Coverage: 100%)
Gene names: JW3266, b3304, rplR
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Large ribosomal subunit protein bL19 Chain: c
Molecule details ›
Chain: c
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7K6 (Residues: 1-115; Coverage: 100%)
Gene names: JW2587, b2606, rplS
Sequence domains: Ribosomal protein L19
Large ribosomal subunit protein bL20 Chain: d
Molecule details ›
Chain: d
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L3 (Residues: 1-118; Coverage: 100%)
Gene names: JW1706, b1716, pdzA, rplT
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein bL21 Chain: e
Molecule details ›
Chain: e
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG48 (Residues: 1-103; Coverage: 100%)
Gene names: JW3153, b3186, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein uL22 Chain: f
Molecule details ›
Chain: f
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P61175 (Residues: 1-110; Coverage: 100%)
Gene names: JW3277, b3315, eryB, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL23 Chain: g
Molecule details ›
Chain: g
Length: 100 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-100; Coverage: 100%)
Gene names: JW3280, b3318, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: h
Molecule details ›
Chain: h
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60624 (Residues: 1-104; Coverage: 100%)
Gene names: JW3271, b3309, rplX
Sequence domains:
Large ribosomal subunit protein bL25 Chain: i
Molecule details ›
Chain: i
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68919 (Residues: 1-94; Coverage: 100%)
Gene names: JW2173, b2185, rplY
Sequence domains: Ribosomal L25p family
Large ribosomal subunit protein bL27 Chain: j
Molecule details ›
Chain: j
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)
Gene names: JW3152, b3185, rpmA
Sequence domains: Ribosomal L27 protein
Large ribosomal subunit protein bL28 Chain: k
Molecule details ›
Chain: k
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M2 (Residues: 1-78; Coverage: 100%)
Gene names: JW3612, b3637, rpmB
Sequence domains: Ribosomal L28 family
Large ribosomal subunit protein uL29 Chain: l
Molecule details ›
Chain: l
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein uL30 Chain: m
Molecule details ›
Chain: m
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG51 (Residues: 1-59; Coverage: 100%)
Gene names: JW3264, b3302, rpmD
Sequence domains: Ribosomal protein L30p/L7e
Large ribosomal subunit protein bL32 Chain: n
Molecule details ›
Chain: n
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N4 (Residues: 1-57; Coverage: 100%)
Gene names: JW1075, b1089, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33 Chain: o
Molecule details ›
Chain: o
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N9 (Residues: 1-55; Coverage: 100%)
Gene names: JW3611, b3636, rpmG
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: p
Molecule details ›
Chain: p
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: q
Molecule details ›
Chain: q
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q1 (Residues: 1-65; Coverage: 100%)
Gene names: JW1707, b1717, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein bL36A Chain: r
Molecule details ›
Chain: r
Length: 55 amino acids
Theoretical weight: 6.32 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Gene names: JW3261, b3299, rpmJ
Sequence domains: Ribosomal protein L36
Large ribosomal subunit protein bL31 Chain: L
Molecule details ›
Chain: L
Length: 70 amino acids
Theoretical weight: 7.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M9 (Residues: 1-70; Coverage: 100%)
Gene names: JW3907, b3936, rpmE
Sequence domains: Ribosomal protein L31
Large ribosomal subunit protein uL1 Chain: C
Molecule details ›
Chain: C
Length: 223 amino acids
Theoretical weight: 23.74 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L0 (Residues: 3-225; Coverage: 95%)
Gene names: JW3947, b3984, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein bL9 Chain: U
Molecule details ›
Chain: U
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R1 (Residues: 1-149; Coverage: 100%)
Gene names: JW4161, b4203, rplI
Sequence domains:
Small ribosomal subunit protein uS2 Chain: 1
Molecule details ›
Chain: 1
Length: 239 amino acids
Theoretical weight: 26.58 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 1-239; Coverage: 99%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: 2
Molecule details ›
Chain: 2
Length: 218 amino acids
Theoretical weight: 24.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 1-218; Coverage: 94%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: 3
Molecule details ›
Chain: 3
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 1-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: 4
Molecule details ›
Chain: 4
Length: 162 amino acids
Theoretical weight: 17.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 4-165; Coverage: 97%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
Small ribosomal subunit protein bS6 Chain: 5
Molecule details ›
Chain: 5
Length: 131 amino acids
Theoretical weight: 15.2 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-131; Coverage: 97%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: 6
Molecule details ›
Chain: 6
Length: 152 amino acids
Theoretical weight: 16.99 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 1-152; Coverage: 85%)
Gene names: JW3303, b3341, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: 7
Molecule details ›
Chain: 7
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 1-130; Coverage: 100%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: 8
Molecule details ›
Chain: 8
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7X3 (Residues: 1-130; Coverage: 100%)
Gene names: JW3199, b3230, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: 9
Molecule details ›
Chain: 9
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 1-103; Coverage: 100%)
Gene names: JW3283, b3321, nusE, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 13.84 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 1-129; Coverage: 100%)
Gene names: JW3259, b3297, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: B
Molecule details ›
Chain: B
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 1-124; Coverage: 100%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: D
Molecule details ›
Chain: D
Length: 118 amino acids
Theoretical weight: 13.1 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S9 (Residues: 1-118; Coverage: 100%)
Gene names: JW3260, b3298, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14 Chain: E
Molecule details ›
Chain: E
Length: 101 amino acids
Theoretical weight: 11.56 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 1-101; Coverage: 100%)
Gene names: JW3269, b3307, rpsN
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: F
Molecule details ›
Chain: F
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: G
Molecule details ›
Chain: G
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: H
Molecule details ›
Chain: H
Length: 84 amino acids
Theoretical weight: 9.69 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 1-84; Coverage: 100%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
30S ribosomal protein S18 Chain: I
Molecule details ›
Chain: I
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS19 Chain: J
Molecule details ›
Chain: J
Length: 92 amino acids
Theoretical weight: 10.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 1-92; Coverage: 100%)
Gene names: JW3278, b3316, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS20 Chain: K
Molecule details ›
Chain: K
Length: 87 amino acids
Theoretical weight: 9.69 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 1-87; Coverage: 100%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein bS21 Chain: s
Molecule details ›
Chain: s
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 1-71; Coverage: 100%)
Gene names: JW3037, b3065, rpsU
Sequence domains: Ribosomal protein S21
Small ribosomal subunit protein bS1 Chain: t
Molecule details ›
Chain: t
Length: 557 amino acids
Theoretical weight: 61.22 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG67 (Residues: 1-557; Coverage: 100%)
Gene names: JW0894, b0911, rpsA, ssyF
Sequence domains: S1 RNA binding domain
Ribosome rescue factor SmrB Chain: v
Molecule details ›
Chain: v
Length: 182 amino acids
Theoretical weight: 20.84 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8B2 (Residues: 1-182; Coverage: 100%)
Gene names: JW2328, b2331, smrB, yfcN
Sequence domains: Smr domain
5S ribosomal RNA Chain: O
Molecule details ›
Chain: O
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Sequence domains: 5S ribosomal RNA
23S ribosomal RNA Chain: N
Molecule details ›
Chain: N
Length: 2903 nucleotides
Theoretical weight: 941.31 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
P-site tRNA Chain: M
Molecule details ›
Chain: M
Length: 75 nucleotides
Theoretical weight: 24.06 KDa
Sequence domains: tRNA
Messenger RNA Chain: x
Molecule details ›
Chain: x
Length: 692 nucleotides
Theoretical weight: 221.44 KDa
16S ribosomal RNA Chain: 0
Molecule details ›
Chain: 0
Length: 1539 nucleotides
Theoretical weight: 498.73 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
A-site tRNA Chain: u
Molecule details ›
Chain: u
Length: 73 nucleotides
Theoretical weight: 23.55 KDa
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.7Å
Relevant EMDB volumes: EMD-13958
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Ribosome-associated quality-control mechanisms from bacteria to humans.
Filbeck et al. (2022)
4 more

2 mentions without citation

Ribosome collisions induce mRNA cleavage and ribosome rescue in bacteria.
Saito et al. (2022)
1 more