› 7qhp

X-ray diffraction
1.82Å resolution

Structure of I-Ag7 with a bound hybrid insulin peptide

Released:
DOI: 10.2210/pdb7qhp/pdb
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PDB entry 7qhp coloured by chain, front view.
PDB entry 7qhp coloured by chain, side view.
PDB entry 7qhp coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural plasticity in I-Ag7 links autoreactivity to hybrid insulin peptides in type I diabetes.
Erausquin E, Serra P, Parras D, Santamaria P, López-Sagaseta J
OpenAccess logo Front Immunol 13 924311 (2022)
PMID: 35967292

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134259 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class II histocompatibility antigen, A-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 23.03 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04228 (Residues: 24-218; Coverage: 84%)
Gene name: H2-Aa
Sequence domains:
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 230 amino acids
Theoretical weight: 26.26 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q31135 (Residues: 21-27, 28-226; Coverage: 84%)
Gene name: H2-Ab1
Sequence domains:
Insulin-1 Chain: T

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2221
Unit cell:
a: 91.996Å b: 108.819Å c: 98.055Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.219
Expression system: Cricetulus griseus

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Citations

1 review citation

Using mass spectrometry to identify neoantigens in autoimmune diseases: The type 1 diabetes example.
Lichti et al. (2023)

1 mention without citation

Structural plasticity in I-Ag7 links autoreactivity to hybrid insulin peptides in type I diabetes.
Erausquin et al. (2022)

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