7qj8

Electron Microscopy
8.7Å resolution

Structure of recombinant human gamma-Tubulin Ring Complex 12-spoked assembly intermediate (spokes 3-14, substoichiometric spokes 1-2)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 35-mer (preferred)
PDBe Complex ID:
PDB-CPX-105653 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Gamma-tubulin complex component 5 Chains: J, l
Molecule details ›
Chains: J, l
Length: 1024 amino acids
Theoretical weight: 118.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q96RT8 (Residues: 1-1024; Coverage: 100%)
Gene names: GCP5, KIAA1899, TUBGCP5
Sequence domains:
Tubulin gamma-1 chain Chains: 1, 2, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, Q, R, S, T, U, V, W, X, Y, Z
Length: 451 amino acids
Theoretical weight: 51.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: P23258 (Residues: 1-451; Coverage: 100%)
Gene names: TUBG, TUBG1
Sequence domains:
Actin, cytoplasmic 1 Chain: e
Molecule details ›
Chain: e
Length: 375 amino acids
Theoretical weight: 41.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A0A6I9HGD1 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Mitotic-spindle organizing protein 1 Chains: b, d, i, k, m
Molecule details ›
Chains: b, d, i, k, m
Length: 82 amino acids
Theoretical weight: 8.49 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q08AG7 (Residues: 1-82; Coverage: 100%)
Gene names: C13orf37, MOZART1, MZT1
Sequence domains: Mitotic-spindle organizing gamma-tubulin ring associated
Gamma-tubulin complex component 3 Chains: D, F, H, N, a, h, j
Molecule details ›
Chains: D, F, H, N, a, h, j
Length: 907 amino acids
Theoretical weight: 103.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q96CW5 (Residues: 1-907; Coverage: 100%)
Gene names: GCP3, TUBGCP3
Sequence domains:
Gamma-tubulin complex component 2 Chains: C, E, G, M
Molecule details ›
Chains: C, E, G, M
Length: 902 amino acids
Theoretical weight: 102.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9BSJ2 (Residues: 1-902; Coverage: 100%)
Gene names: GCP2, TUBGCP2
Sequence domains:
Gamma-tubulin complex component 4 Chains: I, K
Molecule details ›
Chains: I, K
Length: 667 amino acids
Theoretical weight: 76.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9UGJ1 (Residues: 1-667; Coverage: 100%)
Gene names: 76P, GCP4, TUBGCP4
Sequence domains:
Gamma-tubulin complex component 6 Chains: L, c
Molecule details ›
Chains: L, c
Length: 1819 amino acids
Theoretical weight: 200.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q96RT7 (Residues: 1-1819; Coverage: 100%)
Gene names: GCP6, KIAA1669, TUBGCP6
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.7Å
Relevant EMDB volumes: EMD-14013
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017