› 7qjc

Electron Microscopy
16.1Å resolution

Structure of recombinant human gamma-Tubulin Ring Complex (spokes 1-14, homogeneous dataset)

Released:
DOI: 10.2210/pdb7qjc/pdb
PDB entry 7qjc coloured by chain, front view.
PDB entry 7qjc coloured by chain, side view.
PDB entry 7qjc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modular assembly of the principal microtubule nucleator γ-TuRC.
Würtz M, Zupa E, Atorino ES, Neuner A, Böhler A, Rahadian AS, Vermeulen BJA, Tonon G, Eustermann S, Schiebel E, Pfeffer S
OpenAccess logo Nat Commun 13 473 (2022)
PMID: 35078983
Related structures: EMD-14017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 41-mer (preferred)
PDBe Complex ID:
PDB-CPX-105655 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Actin, cytoplasmic 1 Chain: e
Molecule details ›
Chain: e
Length: 375 amino acids
Theoretical weight: 41.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A0A6I9HGD1 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Gamma-tubulin complex component 2 Chains: A, C, E, G, M
Molecule details ›
Chains: A, C, E, G, M
Length: 902 amino acids
Theoretical weight: 102.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9BSJ2 (Residues: 1-902; Coverage: 100%)
Gene names: GCP2, TUBGCP2
Sequence domains:
Gamma-tubulin complex component 3 Chains: B, D, F, H, N, a, f, h, j
Gamma-tubulin complex component 4 Chains: I, K
Gamma-tubulin complex component 5 Chains: J, l
Gamma-tubulin complex component 6 Chains: L, c
Tubulin gamma-1 chain Chains: 1, 2, O, P, Q, R, S, T, U, V, W, X, Y, Z
Mitotic-spindle organizing protein 1 Chains: b, d, g, i, k, m

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 16.1Å
Relevant EMDB volumes: EMD-14017
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017

Quick links

Citations

3 review citations

How Microtubules Build the Spindle Branch by Branch.
Travis et al. (2022)
2 more