7qn8

Electron Microscopy
3.1Å resolution

Cryo-EM structure of human full-length beta3delta GABA(A)R in complex with histamine and nanobody Nb25

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-208035 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Gamma-aminobutyric acid receptor subunit beta-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 473 amino acids
Theoretical weight: 54.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28472 (Residues: 1-473; Coverage: 100%)
Gene name: GABRB3
Sequence domains:
Gamma-aminobutyric acid receptor subunit delta Chain: E
Molecule details ›
Chain: E
Length: 472 amino acids
Theoretical weight: 52.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O14764 (Residues: 1-452; Coverage: 100%)
Gene name: GABRD
Sequence domains:
Nanobody Nb25 Chains: K, L, M

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-14070
Expression system: Homo sapiens