› 7qn8

Electron Microscopy
3.1Å resolution

Cryo-EM structure of human full-length beta3delta GABA(A)R in complex with histamine and nanobody Nb25

Released:
DOI: 10.2210/pdb7qn8/pdb
PDB entry 7qn8 coloured by chain, front view.
PDB entry 7qn8 coloured by chain, side view.
PDB entry 7qn8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Differential assembly diversifies GABAA receptor structures and signalling.
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature 604 190-194 (2022)
PMID: 35355020
Related structures: EMD-14070

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208035 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Gamma-aminobutyric acid receptor subunit beta-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 473 amino acids
Theoretical weight: 54.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Gamma-aminobutyric acid receptor subunit delta Chain: E
Molecule details ›
Chain: E
Length: 472 amino acids
Theoretical weight: 52.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Nanobody Nb25 Chains: K, L, M

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand HSM 3 x HSM
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-14070
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Benzodiazepine Modulation of GABAA Receptors: A Mechanistic Perspective.
Goldschen-Ohm MP. (2022)
7 more

1 mention without citation

Differential assembly diversifies GABAA receptor structures and signalling.
Sente et al. (2022)

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 7qn8 found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-10914
Datasets: 1
Total size: 3000Gb
EMPIAR thumbnail