› 7qng

X-ray diffraction
2.7Å resolution

Structure of a MHC I-Tapasin-ERp57 complex

Released:
DOI: 10.2210/pdb7qng/pdb
Model geometry
Fit model/data
PDB entry 7qng coloured by chain, front view.
PDB entry 7qng coloured by chain, side view.
PDB entry 7qng coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an MHC I-tapasin-ERp57 editing complex defines chaperone promiscuity.
Müller IK, Winter C, Thomas C, Spaapen RM, Trowitzsch S, Tampé R
OpenAccess logo Nat Commun 13 5383 (2022)
PMID: 36104323

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127516 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Tapasin Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 44.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15533 (Residues: 1-401; Coverage: 89%)
Gene names: NGS17, TAPA, TAPBP
Sequence domains: Immunoglobulin C1-set domain
Protein disulfide-isomerase A3 Chain: B
Molecule details ›
Chain: B
Length: 505 amino acids
Theoretical weight: 56.83 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30101 (Residues: 1-505; Coverage: 100%)
Gene names: ERP57, ERP60, GRP58, PDIA3
Sequence domains:
H-2 class I histocompatibility antigen, D-B alpha chain Chain: C
Beta-2-microglobulin Chain: D

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Unit cell:
a: 74.76Å b: 168.53Å c: 187.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.229
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 review citation

Pathways of MHC I cross-presentation of exogenous antigens.
Cruz et al. (2023)
7 other articles

3 mentions without citation

The mode of action of tapasin on major histocompatibility class I (MHC-I) molecules.
Lan et al. (2023)
2 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO