› 7qpl

X-ray diffraction
1.77Å resolution

Crystal structure of phosphoserine phosphatase (SerB) from Brucella melitensis in complex with phosphate and magnesium

Released:
DOI: 10.2210/pdb7qpl/pdb
PDB entry 7qpl coloured by chain, front view.
PDB entry 7qpl coloured by chain, side view.
PDB entry 7qpl coloured by chain, top view.
Source organism: Brucella melitensis bv. 1 str. 16M
Entry authors: Pierson E, Wouters J

Function and Biology Details

Reaction catalysed: 
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine phosphatase Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 32.55 KDa
Source organism: Brucella melitensis bv. 1 str. 16M
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8YI30 (Residues: 2-302; Coverage: 100%)
Gene name: BMEI0615
Sequence domains: haloacid dehalogenase-like hydrolase

Ligands and Environments

6 bound ligands:
Ligand PEG 1 x PEG
Ligand GOL 6 x GOL
Ligand FMT 5 x FMT
Ligand EDO 2 x EDO
Ligand MG 1 x MG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I213
Unit cell:
a: 143.21Å b: 143.21Å c: 143.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.183
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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