› 7qsg

X-ray diffraction
2.75Å resolution

Methylmannose polysaccharide mannosyltransferase from M. hassiacum

Released:
DOI: 10.2210/pdb7qsg/pdb
PDB entry 7qsg coloured by chain, front view.
PDB entry 7qsg coloured by chain, side view.
PDB entry 7qsg coloured by chain, top view.
Source organism: Mycolicibacterium hassiacum
Primary publication:
Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
Maranha A, Costa M, Ripoll-Rozada J, Manso JA, Miranda V, Mendes VM, Manadas B, Macedo-Ribeiro S, Ventura MR, Pereira PJB, Empadinhas N
OpenAccess logo Commun Biol 6 108 (2023)
PMID: 36707645

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MMP alpha-(1->4)-mannosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 437 amino acids
Theoretical weight: 48.28 KDa
Source organism: Mycolicibacterium hassiacum
Expression system: Escherichia coli
UniProt:
  • Canonical: K5BE02 (Residues: 1-411; Coverage: 100%)
Gene names: C731_4166, MHAS_04402, manT
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2
Unit cell:
a: 136.261Å b: 77.4Å c: 101.21Å
α: 90° β: 106.49° γ: 90°
R-values:
R R work R free
0.27 0.269 0.287
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
Maranha et al. (2023)