› 7qss

X-ray diffraction
1.56Å resolution

Crystal structure of homing endonuclease-associated TliVMA intein (C1A)

Released:
DOI: 10.2210/pdb7qss/pdb
PDB entry 7qss coloured by chain, front view.
PDB entry 7qss coloured by chain, side view.
PDB entry 7qss coloured by chain, top view.
Source organism: Thermococcus litoralis
Primary publication:
Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.
Beyer HM, Iwaï H
OpenAccess logo Front Mol Biosci 9 855511 (2022)
PMID: 35372505

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124789 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
V-type ATP synthase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.94 KDa
Source organism: Thermococcus litoralis
Expression system: Escherichia coli
UniProt:
  • Canonical: H3ZKG9 (Residues: 235-666; Coverage: 43%)
Gene names: OCC_09254, atpA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P212121
Unit cell:
a: 48.8Å b: 96.18Å c: 96.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.21
Expression system: Escherichia coli

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Citations

1 review citation

Genome Editing for Sustainable Crop Improvement and Mitigation of Biotic and Abiotic Stresses.
Hamdan et al. (2022)