› 7qst

X-ray diffraction
2.49Å resolution

Crystal structure of homing endonuclease-associated PhoVMA intein (C1A)

Released:
DOI: 10.2210/pdb7qst/pdb
PDB entry 7qst coloured by chain, front view.
PDB entry 7qst coloured by chain, side view.
PDB entry 7qst coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.
Beyer HM, Iwaï H
OpenAccess logo Front Mol Biosci 9 855511 (2022)
PMID: 35372505

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129601 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-Pho2 Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 42.58 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O57728 (Residues: 238-616; Coverage: 39%)
Gene names: PH1975, atpA
Sequence domains: Intein splicing domain

Ligands and Environments

2 bound ligands:
Ligand CD 2 x CD
Ligand NI 3 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 91.08Å b: 81.87Å c: 67.69Å
α: 90° β: 112.85° γ: 90°
R-values:
R R work R free
0.186 0.183 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Genome Editing for Sustainable Crop Improvement and Mitigation of Biotic and Abiotic Stresses.
Hamdan et al. (2022)