› 7qx3

X-ray diffraction
3.6Å resolution

Structure of the transaminase TR2E2 with EOS

Released:
DOI: 10.2210/pdb7qx3/pdb
PDB entry 7qx3 coloured by chain, front view.
PDB entry 7qx3 coloured by chain, side view.
PDB entry 7qx3 coloured by chain, top view.
Source organism: Acidihalobacter
Primary publication:
A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.
Roda S, Fernandez-Lopez L, Benedens M, Bollinger A, Thies S, Schumacher J, Coscolín C, Kazemi M, Santiago G, Gertzen CGW, Gonzalez-Alfonso JL, Plou FJ, Jaeger KE, Smits SHJ, Ferrer M, Guallar V
OpenAccess logo Angew Chem Int Ed Engl 61 e202207344 (2022)
PMID: 35734849

Function and Biology Details

Reaction catalysed: 
L-alanine + 3-oxopropanoate = pyruvate + beta-alanine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226614 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase TR2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 465 amino acids
Theoretical weight: 51.66 KDa
Source organism: Acidihalobacter
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3G5BC54 (Residues: 1-457; Coverage: 100%)
Sequence domains: Aminotransferase class-III

Ligands and Environments

1 bound ligand:
Ligand GH0 4 x GH0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 100.61Å b: 109.502Å c: 211.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.218
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.
Roda et al. (2022)
8 more