› 7qzk

X-ray diffraction
1.66Å resolution

Crystal structure of mouse CNPase catalytic domain, V318I mutant

Released:
DOI: 10.2210/pdb7qzk/pdb
PDB entry 7qzk coloured by chain, front view.
PDB entry 7qzk coloured by chain, side view.
PDB entry 7qzk coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Markusson S, Kursula P

Function and Biology Details

Reaction catalysed: 
Nucleoside 2',3'-cyclic phosphate + H(2)O = nucleoside 2'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2',3'-cyclic-nucleotide 3'-phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.31 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P16330 (Residues: 179-398; Coverage: 52%)
Gene names: Cnp, Cnp1
Sequence domains: 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP or CNPase)

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 39.53Å b: 47.83Å c: 51.18Å
α: 90° β: 97.21° γ: 90°
R-values:
R R work R free
0.179 0.174 0.222
Expression system: Escherichia coli

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