› 7r1h

X-ray diffraction
1.96Å resolution

Crystal structure of a flavodiiron protein D52K mutant in the reduced state from Escherichia coli

Released:
DOI: 10.2210/pdb7r1h/pdb
PDB entry 7r1h coloured by chain, front view.
PDB entry 7r1h coloured by chain, side view.
PDB entry 7r1h coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Borges PT, Teixeira M, Romao CV, Frazao C

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-559477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anaerobic nitric oxide reductase flavorubredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 479 amino acids
Theoretical weight: 54.31 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46877 (Residues: 1-479; Coverage: 100%)
Gene names: JW2680, b2710, flrD, norV, ygaI, ygaJ, ygaK
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand FMN 2 x FMN
Ligand FE 4 x FE
Ligand O 2 x O
Ligand OXY 1 x OXY
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Unit cell:
a: 88.851Å b: 64.787Å c: 146.56Å
α: 90° β: 91.526° γ: 90°
R-values:
R R work R free
0.201 0.201 0.24
Expression system: Escherichia coli

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