› 7r1z

X-ray diffraction
1.94Å resolution

C-terminal domain of hArc in complex with nanobodies H11 and C11, collapsed crystal form

Released:
DOI: 10.2210/pdb7r1z/pdb
PDB entry 7r1z coloured by chain, front view.
PDB entry 7r1z coloured by chain, side view.
PDB entry 7r1z coloured by chain, top view.
Source organisms:
Primary publication:
High-affinity anti-Arc nanobodies provide tools for structural and functional studies.
Markússon S, Hallin EI, Bustad HJ, Raasakka A, Xu J, Muruganandam G, Loris R, Martinez A, Bramham CR, Kursula P
OpenAccess logo PLoS One 17 e0269281 (2022)
PMID: 35671319

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212504 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
NbArc-H11 Chain: E
Molecule details ›
Chain: E
Length: 128 amino acids
Theoretical weight: 14.08 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)
Activity-regulated cytoskeleton-associated protein Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 21.74 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7LC44 (Residues: 206-361; Coverage: 39%)
Gene names: ARC, KIAA0278
Sequence domains:
NbArc-C11 Chain: C
Molecule details ›
Chain: C
Length: 120 amino acids
Theoretical weight: 13.13 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21212
Unit cell:
a: 66.26Å b: 139.29Å c: 43.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Molecular physiology of Arc/Arg3.1: The oligomeric state hypothesis of synaptic plasticity.
Eriksen et al. (2022)
3 other articles

2 mentions without citation

Structural basis of purine nucleotide inhibition of human uncoupling protein 1.
Jones et al. (2023)
1 more