› 7r2g

X-ray diffraction
1.98Å resolution

USP15 D1D2 in catalytically-competent state bound to mitoxantrone stack (isoform 2)

Released:
DOI: 10.2210/pdb7r2g/pdb
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PDB entry 7r2g coloured by chain, front view.
PDB entry 7r2g coloured by chain, side view.
PDB entry 7r2g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mitoxantrone stacking does not define the active or inactive state of USP15 catalytic domain.
Priyanka A, Tisi D, Sixma TK
J Struct Biol 214 107862 (2022)
PMID: 35605756

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 15 Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 41.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y4E8 (Residues: 284-948; Coverage: 36%)
Gene names: KIAA0529, USP15
Sequence domains: Ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
Ligand MIX 12 x MIX
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 63.882Å b: 97.399Å c: 62.995Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.206 0.204 0.238
Expression system: Escherichia coli BL21(DE3)

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