› 7r30

X-ray diffraction
1.9Å resolution

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with ADP and AMP resulting from ADP soak

Released:
DOI: 10.2210/pdb7r30/pdb
PDB entry 7r30 coloured by chain, front view.
PDB entry 7r30 coloured by chain, side view.
PDB entry 7r30 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA.
Selim KA, Haffner M, Mantovani O, Albrecht R, Zhu H, Hagemann M, Forchhammer K, Hartmann MD
OpenAccess logo Proc Natl Acad Sci U S A 120 e2205882120 (2023)
PMID: 36800386

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-associated protein slr1513 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 120 amino acids
Theoretical weight: 13.22 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P73954 (Residues: 1-110; Coverage: 100%)
Gene name: slr1513
Sequence domains: Nitrogen regulatory protein P-II

Ligands and Environments

3 bound ligands:
Ligand BCT 1 x BCT
Ligand ADP 3 x ADP
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P32
Unit cell:
a: 63.637Å b: 63.637Å c: 82.207Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.194
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Roles of second messengers in the regulation of cyanobacterial physiology: the carbon-concentrating mechanism and beyond.
Mantovani et al. (2023)
1 more

1 mention without citation

Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA.
Selim et al. (2023)