› 7r31

X-ray diffraction
1.52Å resolution

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803, C105A+C110A variant, in complex with ATP (co-crystal), tetragonal crystal form

Released:
DOI: 10.2210/pdb7r31/pdb
PDB entry 7r31 coloured by chain, front view.
PDB entry 7r31 coloured by chain, side view.
PDB entry 7r31 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA.
Selim KA, Haffner M, Mantovani O, Albrecht R, Zhu H, Hagemann M, Forchhammer K, Hartmann MD
OpenAccess logo Proc Natl Acad Sci U S A 120 e2205882120 (2023)
PMID: 36800386

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-associated protein slr1513 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 120 amino acids
Theoretical weight: 13.16 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P73954 (Residues: 1-110; Coverage: 100%)
Gene name: slr1513
Sequence domains: Nitrogen regulatory protein P-II

Ligands and Environments

4 bound ligands:
Ligand ATP 3 x ATP
Ligand NA 3 x NA
Ligand CL 1 x CL
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41
Unit cell:
a: 73.19Å b: 73.19Å c: 89.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.206
Expression system: Escherichia coli

Quick links

Citations

2 mentions without citation

Roles of second messengers in the regulation of cyanobacterial physiology: the carbon-concentrating mechanism and beyond.
Mantovani et al. (2023)
1 more