› 7r32

X-ray diffraction
1.75Å resolution

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803, delta104 variant, in complex with ADP (co-crystal), tetragonal crystal form

Released:
DOI: 10.2210/pdb7r32/pdb
PDB entry 7r32 coloured by chain, front view.
PDB entry 7r32 coloured by chain, side view.
PDB entry 7r32 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA.
Selim KA, Haffner M, Mantovani O, Albrecht R, Zhu H, Hagemann M, Forchhammer K, Hartmann MD
Proc Natl Acad Sci U S A 120 e2205882120 (2023)
PMID: 36800386

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-associated protein slr1513 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.68 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P73954 (Residues: 1-104; Coverage: 95%)
Gene name: slr1513
Sequence domains: Nitrogen regulatory protein P-II

Ligands and Environments

1 bound ligand:
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41
Unit cell:
a: 73.98Å b: 73.98Å c: 88.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.209
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Roles of second messengers in the regulation of cyanobacterial physiology: the carbon-concentrating mechanism and beyond.
Mantovani et al. (2023)

1 mention without citation

Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA.
Selim et al. (2023)