› 7r36

X-ray diffraction
2.2Å resolution

Difference-refined structure of fatty acid photodecarboxylase 2 microsecond following 400-nm laser irradiation of the dark-state determined by SFX

Released:
DOI: 10.2210/pdb7r36/pdb
Model geometry
Fit model/data
PDB entry 7r36 coloured by chain, front view.
PDB entry 7r36 coloured by chain, side view.
PDB entry 7r36 coloured by chain, top view.
Source organism: Chlorella variabilis
Primary publication:
Time-resolved serial femtosecond crystallography on fatty-acid photodecarboxylase: lessons learned.
Hadjidemetriou K, Coquelle N, Barends TRM, De Zitter E, Schlichting I, Colletier JP, Weik M
OpenAccess logo Acta Crystallogr D Struct Biol 78 1131-1142 (2022)
PMID: 36048153

Function and Biology Details

Reaction catalysed: 
A long-chain fatty acid + light = a long-chain alkane + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103997 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid photodecarboxylase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 578 amino acids
Theoretical weight: 61.12 KDa
Source organism: Chlorella variabilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A248QE08 (Residues: 76-654; Coverage: 88%)
Gene names: CHLNCDRAFT_28598, FAP
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand STE 4 x STE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P21
Unit cell:
a: 61.39Å b: 60.01Å c: 182.9Å
α: 90° β: 90.6° γ: 90°
R-values:
R R work R free
0.301 0.297 0.379
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Photoenzymatic Decarboxylation to Produce Hydrocarbon Fuels: A Critical Review.
Sui et al. (2023)

1 mention without citation

Time-resolved serial femtosecond crystallography on fatty-acid photodecarboxylase: lessons learned.
Hadjidemetriou et al. (2022)

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO