› 7r7e

X-ray diffraction
1.99Å resolution

Synechococcus Olefin Synthase FAAL domain in complex with AMP and pyrophosphate

Released:
DOI: 10.2210/pdb7r7e/pdb
PDB entry 7r7e coloured by chain, front view.
PDB entry 7r7e coloured by chain, side view.
PDB entry 7r7e coloured by chain, top view.
Source organism: Synechococcus sp. PCC 7002
Entry authors: Sikkema AP, Strugis RM, Smith JL

Function and Biology Details

Reaction catalysed: 
ATP + a long-chain fatty acid + an [acyl-carrier-protein] = AMP + diphosphate + a long-chain acyl-[acyl-carrier-protein]
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 572 amino acids
Theoretical weight: 61.99 KDa
Source organism: Synechococcus sp. PCC 7002
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1XKC6 (Residues: 2-570; Coverage: 21%)
Gene name: SYNPCC7002_A1173
Sequence domains: AMP-binding enzyme

Ligands and Environments

4 bound ligands:
Ligand AMP 2 x AMP
Ligand POP 2 x POP
Ligand ACT 2 x ACT
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 201.288Å b: 60.92Å c: 95.929Å
α: 90° β: 101.175° γ: 90°
R-values:
R R work R free
0.198 0.196 0.239
Expression system: Escherichia coli BL21(DE3)

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