› 7rab

X-ray diffraction
1.92Å resolution

Crystal structure of a dodecameric multicopper oxidase from M. hydrothermalis in a cubic lattice

Released:
DOI: 10.2210/pdb7rab/pdb
PDB entry 7rab coloured by chain, front view.
PDB entry 7rab coloured by chain, side view.
PDB entry 7rab coloured by chain, top view.
Source organism: Marinithermus hydrothermalis
Primary publication:
Crystal structures of a dodecameric multicopper oxidase from Marinithermus hydrothermalis.
Paavola JL, Battistin U, Ogata CM, Georgiadis MM
Acta Crystallogr D Struct Biol 77 1336-1345 (2021)
PMID: 34605435

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-514324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 348 amino acids
Theoretical weight: 39.42 KDa
Source organism: Marinithermus hydrothermalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2NNS0 (Residues: 32-359; Coverage: 100%)
Gene name: Marky_0543
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CU 16 x CU
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P213
Unit cell:
a: 224.241Å b: 224.241Å c: 224.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.27 0.298
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Structure-function studies of a novel laccase-like multicopper oxidase from Thermothelomyces thermophila provide insights into its biological role.
Kosinas et al. (2023)
1 more

1 mention without citation

Crystal structures of a dodecameric multicopper oxidase from Marinithermus hydrothermalis.
Paavola et al. (2021)