7rai

Electron Microscopy
3.24Å resolution

Cryo-EM structure of M4008_N1 Fab in complex with BG505 DS-SOSIP.664 Env trimer

Released:
Primary publication:
A site of vulnerability at V3 crown defined by HIV-1 bNAb M4008_N1.
OpenAccess logo Nat Commun 12 6464 (2021)
PMID: 34753944
Related structures: EMD-24362

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-212184 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (8 distinct):
Envelope glycoprotein gp160 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 476 amino acids
Theoretical weight: 53.3 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
Transmembrane protein gp41 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 153 amino acids
Theoretical weight: 17.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
M4008_N1 Fab heavy chain Chains: H, I, J
M4008_N1 Fab light chain Chains: L, M, N

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.24Å
Relevant EMDB volumes: EMD-24362
Expression system: Homo sapiens