› 7rdu

X-ray diffraction
2.5Å resolution

Crystal structure of Campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH

Released:
DOI: 10.2210/pdb7rdu/pdb
PDB entry 7rdu coloured by chain, front view.
PDB entry 7rdu coloured by chain, side view.
PDB entry 7rdu coloured by chain, top view.
Source organism: Campylobacter jejuni RM1221
Entry authors: Guddat LW, You L

Function and Biology Details

Reaction catalysed: 
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketol-acid reductoisomerase (NADP(+)) Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 35.74 KDa
Source organism: Campylobacter jejuni RM1221
Expression system: Escherichia
UniProt:
  • Canonical: Q5HVD9 (Residues: 2-330; Coverage: 97%)
Gene names: CJE0735, ilvC
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP

Cofactor: Ligand NDP 1 x NDP
1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: I23
Unit cell:
a: 130.108Å b: 130.108Å c: 130.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.195 0.256
Expression system: Escherichia

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