› 7rec

X-ray diffraction
2.2Å resolution

Structure of Thr354Asn, Glu355Gln, Thr412Asn, Ile414Met, Ile464His, and Phe467Met mutant human CaMKII alpha hub bound to 5-HDC

Released:
DOI: 10.2210/pdb7rec/pdb
PDB entry 7rec coloured by chain, front view.
PDB entry 7rec coloured by chain, side view.
PDB entry 7rec coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
GHB analogs confer neuroprotection through specific interaction with the CaMKIIα hub domain.
Leurs U, Klein AB, McSpadden ED, Griem-Krey N, Solbak SMØ, Houlton J, Villumsen IS, Vogensen SB, Hamborg L, Gauger SJ, Palmelund LB, Larsen ASG, Shehata MA, Kelstrup CD, Olsen JV, Bach A, Burnie RO, Kerr DS, Gowing EK, Teurlings SMW, Chi CC, Gee CL, Frølund B, Kornum BR, van Woerden GM, Clausen RP, Kuriyan J, Clarkson AN, Wellendorph P
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 34330837

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium/calmodulin-dependent protein kinase type II subunit alpha Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 135 amino acids
Theoretical weight: 15.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UQM7 (Residues: 345-475; Coverage: 27%)
Gene names: CAMK2A, CAMKA, KIAA0968
Sequence domains: Calcium/calmodulin dependent protein kinase II association domain

Ligands and Environments

2 bound ligands:
Ligand 7ZV 6 x 7ZV
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 101.433Å b: 182.958Å c: 106.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Role of Ca2+/Calmodulin-Dependent Protein Kinase Type II in Mediating Function and Dysfunction at Glutamatergic Synapses.
Mohanan et al. (2022)
2 more