› 7rf0

X-ray diffraction
1.59Å resolution

MYCOBACTERIUM ABSCESSUS TRNA METHYLTRANSFERASE IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND MAGNESIUM

Released:
DOI: 10.2210/pdb7rf0/pdb
PDB entry 7rf0 coloured by chain, front view.
PDB entry 7rf0 coloured by chain, side view.
PDB entry 7rf0 coloured by chain, top view.
Source organism: Mycobacteroides abscessus
Entry authors: Prucha GR, Ismail M, Suske A, Das B, Oz M, Perez A, Bolen R, Jayaraman S, Stojanoff V, Halloran J

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine-N(1)-)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 25.47 KDa
Source organism: Mycobacteroides abscessus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1M9INC4 (Residues: 1-232; Coverage: 96%)
Gene names: SAMEA2070793_01157, trmD
Sequence domains: tRNA (Guanine-1)-methyltransferase

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 74.398Å b: 78.835Å c: 86.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.213
Expression system: Escherichia coli

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