› 7rf9

X-ray diffraction
1.93Å resolution

O2-, PLP-dependent desaturase Plu4 intermediate-bound enzyme

Released:
DOI: 10.2210/pdb7rf9/pdb
PDB entry 7rf9 coloured by chain, front view.
PDB entry 7rf9 coloured by chain, side view.
PDB entry 7rf9 coloured by chain, top view.
Source organism: Pseudoalteromonas luteoviolacea
Primary publication:
A shared mechanistic pathway for pyridoxal phosphate-dependent arginine oxidases.
Hoffarth ER, Caddell Haatveit K, Kuatsjah E, MacNeil GA, Saroya S, Walsby CJ, Eltis LD, Houk KN, Garcia-Borràs M, Ryan KS
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34580201

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 44.64 KDa
Source organism: Pseudoalteromonas luteoviolacea
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0C1MLE8 (Residues: 1-381; Coverage: 100%)
Gene name: JF50_03865
Sequence domains: Aminotransferase class I and II

Ligands and Environments

6 bound ligands:
Ligand BTB 5 x BTB
Ligand ACT 5 x ACT
Ligand EJ1 4 x EJ1
Ligand GOL 1 x GOL
Ligand EDO 19 x EDO
Ligand AE3 1 x AE3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 74.922Å b: 70.279Å c: 138.568Å
α: 90° β: 98.11° γ: 90°
R-values:
R R work R free
0.153 0.151 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

On the Evolutionary History of the Twenty Encoded Amino Acids.
Kirschning A. (2022)
1 other article

2 mentions without citation

Re-creation of historical chrysotile-containing joint compounds.
Brorby et al. (2008)
1 more