› 7rfe

Electron Microscopy
2.6Å resolution

HUMAN IMPDH1 TREATED WITH GTP, IMP, AND NAD+; INTERFACE-CENTERED

Released:
DOI: 10.2210/pdb7rfe/pdb
PDB entry 7rfe coloured by chain, front view.
PDB entry 7rfe coloured by chain, side view.
PDB entry 7rfe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
IMPDH1 retinal variants control filament architecture to tune allosteric regulation.
Burrell AL, Nie C, Said M, Simonet JC, Fernández-Justel D, Johnson MC, Quispe J, Buey RM, Peterson JR, Kollman JM
Nat Struct Mol Biol 29 47-58 (2022)
PMID: 35013599
Related structures: EMD-24439

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 504 amino acids
Theoretical weight: 54.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20839 (Residues: 11-514; Coverage: 98%)
Gene names: IMPD1, IMPDH1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 16 x GTP
Ligand ATP 8 x ATP
Ligand IMP 8 x IMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-24439
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

IMPDH dysregulation in disease: a mini review.
Burrell et al. (2022)
5 more

2 mentions without citation

IMPDH1 retinal variants control filament architecture to tune allosteric regulation.
Burrell et al. (2022)
1 more