› 7rfv

X-ray diffraction
3.2Å resolution

Tailspike protein 4 (TSP4) from phage CBA120, residues 1-250, obtained in the presence of PEG8000

Released:
DOI: 10.2210/pdb7rfv/pdb
PDB entry 7rfv coloured by chain, front view.
PDB entry 7rfv coloured by chain, side view.
PDB entry 7rfv coloured by chain, top view.
Source organism: Escherichia virus CBA120
Primary publication:
Structure of Escherichia coli O157:H7 bacteriophage CBA120 tailspike protein 4 baseplate anchor and tailspike assembly domains (TSP4-N).
Chao KL, Shang X, Greenfield J, Linden SB, Alreja AB, Nelson DC, Herzberg O
OpenAccess logo Sci Rep 12 2061 (2022)
PMID: 35136138

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tail spike TSP1/Gp66 N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 26.67 KDa
Source organism: Escherichia virus CBA120
Expression system: Escherichia coli
UniProt:
  • Canonical: G3M192 (Residues: 1-250; Coverage: 24%)
Gene name: orf213

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R32
Unit cell:
a: 84.683Å b: 84.683Å c: 225.073Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.201 0.301
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Evolutionary Dynamics between Phages and Bacteria as a Possible Approach for Designing Effective Phage Therapies against Antibiotic-Resistant Bacteria.
Hasan et al. (2022)
4 other articles

1 mention without citation

Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility.
Islam et al. (2023)