› 7rhr

Electron Microscopy
3Å resolution

Cryo-EM structure of Xenopus Patched-1 in nanodisc

Released:
DOI: 10.2210/pdb7rhr/pdb
PDB entry 7rhr coloured by chain, front view.
PDB entry 7rhr coloured by chain, side view.
PDB entry 7rhr coloured by chain, top view.
Source organism: Xenopus calcaratus
Primary publication:
Structural basis for catalyzed assembly of the Sonic hedgehog-Patched1 signaling complex.
Huang P, Wierbowski BM, Lian T, Chan C, García-Linares S, Jiang J, Salic A
Dev Cell 57 670-685.e8 (2022)
PMID: 35231446
Related structures: EMD-24467

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
SSD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 1205 amino acids
Theoretical weight: 134.62 KDa
Source organism: Xenopus calcaratus
Expression system: Insecta environmental sample
UniProt:
  • Canonical: Q98SW6 (Residues: 1-1178; Coverage: 83%)
Gene names: Ptc-1, Ptc1, X-ptc1, XPtc1, patched, patched1, ptc1, ptch1, ptch1.S
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 2 x NAG
2 bound ligands:
Ligand CLR 3 x CLR
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-24467
Expression system: Insecta environmental sample

Quick links

Citations

4 review citations

Cellular and molecular mechanisms of Hedgehog signalling.
Zhang et al. (2023)
3 more

1 mention without citation

Structural basis for catalyzed assembly of the Sonic hedgehog-Patched1 signaling complex.
Huang et al. (2022)