› 7ri4

Electron Microscopy
3.4Å resolution

Structure of a BAM/EspP(beta9-12) hybrid-barrel intermediate

Released:
DOI: 10.2210/pdb7ri4/pdb
PDB entry 7ri4 coloured by chain, front view.
PDB entry 7ri4 coloured by chain, side view.
PDB entry 7ri4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Plasticity within the barrel domain of BamA mediates a hybrid-barrel mechanism by BAM.
Wu R, Bakelar JW, Lundquist K, Zhang Z, Kuo KM, Ryoo D, Pang YT, Sun C, White T, Klose T, Jiang W, Gumbart JC, Noinaj N
Nat Commun 12 7131 (2021)
PMID: 34880256
Related structures: EMD-24473

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-128464 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Autotransporter protein EspP translocator Chain: G
Molecule details ›
Chain: G
Length: 83 amino acids
Theoretical weight: 8.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: O32591 (Residues: 1218-1300; Coverage: 7%)
Gene names: espP, pssA
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 810 amino acids
Theoretical weight: 90.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 1-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 39.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77774 (Residues: 22-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 344 amino acids
Theoretical weight: 36.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A903 (Residues: 1-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC02 (Residues: 1-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 123 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A937 (Residues: 1-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-24473
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Surveying membrane landscapes: a new look at the bacterial cell surface.
Lithgow et al. (2023)
4 more