› 7rin

X-ray diffraction
1.85Å resolution

Apo PTP1B by Native S-SAD at Room Temperature

Released:
DOI: 10.2210/pdb7rin/pdb
PDB entry 7rin coloured by chain, front view.
PDB entry 7rin coloured by chain, side view.
PDB entry 7rin coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Native SAD phasing at room temperature.
Greisman JB, Dalton KM, Sheehan CJ, Klureza MA, Kurinov I, Hekstra DR
Acta Crystallogr D Struct Biol 78 986-996 (2022)
PMID: 35916223

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase

Ligands and Environments

1 bound ligand:
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 89.538Å b: 89.538Å c: 106.144Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.133 0.132 0.154
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

A unifying Bayesian framework for merging X-ray diffraction data.
Dalton et al. (2022)
4 more

4 mentions without citation

Native SAD phasing at room temperature.
Greisman et al. (2022)
3 more