› 7rl5

Electron Microscopy
3.8Å resolution

Yeast CTP Synthase (URA8) filament bound to CTP at low pH

Released:
DOI: 10.2210/pdb7rl5/pdb
PDB entry 7rl5 coloured by chain, front view.
PDB entry 7rl5 coloured by chain, side view.
PDB entry 7rl5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen JM, Horowitz A, Lynch EM, Farrell DP, Quispe J, DiMaio F, Kollman JM
OpenAccess logo Elife 10 (2021)
PMID: 34734801
Related structures: EMD-24516

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase Chains: A, B, C, D, E, L, M, O, P, Q, R, S
Molecule details ›
Chains: A, B, C, D, E, L, M, O, P, Q, R, S
Length: 559 amino acids
Theoretical weight: 62.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6A5PYW3 (Residues: 1-571; Coverage: 97%)
Gene names: GI527_G0003359, URA8
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CTP 24 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-24516
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Greater than the sum of parts: Mechanisms of metabolic regulation by enzyme filaments.
Hvorecny et al. (2023)
2 more

1 mention without citation

Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen et al. (2021)