› 7rl8

X-ray diffraction
1.95Å resolution

Crystal Structure of C79A Mutant of Class D beta-lactamase from Clostridium difficile 630

Released:
DOI: 10.2210/pdb7rl8/pdb
PDB entry 7rl8 coloured by chain, front view.
PDB entry 7rl8 coloured by chain, side view.
PDB entry 7rl8 coloured by chain, top view.
Source organism: Clostridioides difficile 630
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Rosas-Lemus M, Jedrzejczak R, Satchell KJF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 29.28 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q188Q3 (Residues: 40-290; Coverage: 87%)
Gene name: CD630_04580
Sequence domains: Penicillin binding protein transpeptidase domain

Ligands and Environments

2 bound ligands:
Ligand PEG 1 x PEG
Ligand SO4 2 x SO4
1 modified residue:
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 86.118Å b: 93.878Å c: 138.346Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links