› 7rmc

Electron Microscopy
3.5Å resolution

Yeast CTP Synthase (Ura7) filament bound to CTP at low pH

Released:
DOI: 10.2210/pdb7rmc/pdb
PDB entry 7rmc coloured by chain, front view.
PDB entry 7rmc coloured by chain, side view.
PDB entry 7rmc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen JM, Horowitz A, Lynch EM, Farrell DP, Quispe J, DiMaio F, Kollman JM
OpenAccess logo Elife 10 (2021)
PMID: 34734801
Related structures: EMD-24560

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase 1 Chains: D, E, F, Q, R, S, T, U, V, W, g, h
Molecule details ›
Chains: D, E, F, Q, R, S, T, U, V, W, g, h
Length: 561 amino acids
Theoretical weight: 62.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P28274 (Residues: 1-572; Coverage: 97%)
Gene names: URA7, YBL039C, YBL0410
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CTP 24 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-24560
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Greater than the sum of parts: Mechanisms of metabolic regulation by enzyme filaments.
Hvorecny et al. (2023)
2 more

1 mention without citation

Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen et al. (2021)