› 7rmk

Electron Microscopy
6.6Å resolution

Yeast CTP Synthase (Ura7) Bundle bound to substrates at low pH

Released:
DOI: 10.2210/pdb7rmk/pdb
PDB entry 7rmk coloured by chain, front view.
PDB entry 7rmk coloured by chain, side view.
PDB entry 7rmk coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen JM, Horowitz A, Lynch EM, Farrell DP, Quispe J, DiMaio F, Kollman JM
Elife 10 (2021)
PMID: 34734801
Related structures: EMD-24575

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b
Length: 553 amino acids
Theoretical weight: 61.88 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A7I9CFN1 (Residues: 1-572; Coverage: 96%)
Gene names: GI527_G0000161, URA7
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 28 x ATP
Ligand UTP 28 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-24575
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Greater than the sum of parts: Mechanisms of metabolic regulation by enzyme filaments.
Hvorecny et al. (2023)
2 more

2 mentions without citation

Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen et al. (2021)
1 more