› 7rmv

Electron Microscopy
6.7Å resolution

Yeast CTP Synthase (Ura7) H360R Filament bound to Substrates

Released:
DOI: 10.2210/pdb7rmv/pdb
PDB entry 7rmv coloured by chain, front view.
PDB entry 7rmv coloured by chain, side view.
PDB entry 7rmv coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen JM, Horowitz A, Lynch EM, Farrell DP, Quispe J, DiMaio F, Kollman JM
OpenAccess logo Elife 10 (2021)
PMID: 34734801
Related structures: EMD-24578

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 553 amino acids
Theoretical weight: 61.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P28274 (Residues: 1-572; Coverage: 96%)
Gene names: URA7, YBL039C, YBL0410
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 8 x ATP
Ligand UTP 8 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.7Å
Relevant EMDB volumes: EMD-24578
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Greater than the sum of parts: Mechanisms of metabolic regulation by enzyme filaments.
Hvorecny et al. (2023)
2 more

1 mention without citation

Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
Hansen et al. (2021)