› 7rn8

X-ray diffraction
1.88Å resolution

Function and Biology Details

Reaction catalysed: 
Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154749 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Caspase-3 subunit p17 Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 16.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42574 (Residues: 34-174; Coverage: 51%)
Gene names: CASP3, CPP32
Sequence domains: Caspase domain
Caspase-3 subunit p12 Chains: B, D
Molecule details ›
Chains: B, D
Length: 95 amino acids
Theoretical weight: 11.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42574 (Residues: 184-277; Coverage: 34%)
Gene names: CASP3, CPP32
Sequence domains: Caspase domain
Ac-VD(Orn)VD-CHO Chains: F, G
Molecule details ›
Chains: F, G
Length: 6 amino acids
Theoretical weight: 571 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand ORN 2 x ORN
Ligand ASA 2 x ASA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 50.266Å b: 67.626Å c: 82.366Å
α: 90° β: 91.042° γ: 90°
R-values:
R R work R free
0.187 0.186 0.214
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Apoptotic cell death in disease-Current understanding of the NCCD 2023.
Vitale et al. (2023)
2 more