› 7roj

X-ray diffraction
1.6Å resolution

Amyloid-related segment of alphaB-crystallin residues 90-100 with G95W mutation

Released:
DOI: 10.2210/pdb7roj/pdb
PDB entry 7roj coloured by chain, front view.
PDB entry 7roj coloured by chain, side view.
PDB entry 7roj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Atomic view of an amyloid dodecamer exhibiting selective cellular toxic vulnerability in acute brain slices.
Gray ALH, Sawaya MR, Acharyya D, Lou J, Edington EM, Best MD, Prosser RA, Eisenberg DS, Do TD
Protein Sci 31 716-727 (2022)
PMID: 34954854

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-crystallin B chain Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 11 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02511 (Residues: 90-100; Coverage: 6%)
Gene names: CRYA2, CRYAB, HSPB5

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand TFA 4 x TFA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 25.27Å b: 36.33Å c: 64.13Å
α: 90° β: 91.92° γ: 90°
R-values:
R R work R free
0.243 0.239 0.277
Expression system: Not provided

Quick links

Citations

1 citations in other articles

Elucidating the Oligomerization and Cellular Interactions of a Trimer Derived from Aβ through Fluorescence and Mass Spectrometric Studies.
Guaglianone et al. (2022)

1 mention without citation

Atomic view of an amyloid dodecamer exhibiting selective cellular toxic vulnerability in acute brain slices.
Gray et al. (2022)