Function and Biology Details
Reaction catalysed:
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Proliferating cell nuclear antigen, PCNA
- DNA clamp superfamily
- Proliferating cell nuclear antigen, PCNA, N-terminal
- Nucleic acid-binding, OB-fold
- DNA ligase, ATP-dependent, conserved site
- DNA ligase, ATP-dependent, central
- DNA ligase, ATP-dependent, N-terminal domain superfamily
- Proliferating cell nuclear antigen, PCNA, C-terminal
6 more domains
Structure analysis Details
Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-121928 (preferred)
Entry contents:
4 distinct polypeptide molecules
3 distinct DNA molecules
3 distinct DNA molecules
Macromolecules (7 distinct):
DNA polymerase sliding clamp 1
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.71 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Length: 258 amino acids
Theoretical weight: 28.71 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
- Canonical:
P57766 (Residues: 2-249; Coverage: 100%)
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
DNA polymerase sliding clamp 2
Molecule details ›
Chain: B
Length: 245 amino acids
Theoretical weight: 27.46 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 245 amino acids
Theoretical weight: 27.46 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
- Canonical: Q97Z84 (Residues: 1-245; Coverage: 100%)
Sequence domains:
DNA polymerase sliding clamp 3
Molecule details ›
DNA ligase
Molecule details ›
Chain: E
Length: 621 amino acids
Theoretical weight: 70 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 621 amino acids
Theoretical weight: 70 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
- Canonical: Q980T8 (Residues: 1-601; Coverage: 100%)
Sequence domains:
