› 7rqf

X-ray diffraction
3.5Å resolution

Crystal Structure of LbcA (lipoprotein binding partner of CtpA) of Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb7rqf/pdb
PDB entry 7rqf coloured by chain, front view.
PDB entry 7rqf coloured by chain, side view.
PDB entry 7rqf coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Pseudomonas aeruginosa C-Terminal Processing Protease CtpA Assembles into a Hexameric Structure That Requires Activation by a Spiral-Shaped Lipoprotein-Binding Partner.
Hsu HC, Wang M, Kovach A, Darwin AJ, Li H
OpenAccess logo mBio e0368021 (2022)
PMID: 35038915

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154890 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TPR repeat-containing protein PA4667 Chains: A, B
Molecule details ›
Chains: A, B
Length: 540 amino acids
Theoretical weight: 61.67 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42810 (Residues: 65-590; Coverage: 89%)
Gene name: PA4667
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 120.699Å b: 120.699Å c: 221.943Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.245 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Bacterial Carboxyl-Terminal Processing Proteases Play Critical Roles in the Cell Envelope and Beyond.
Sommerfield et al. (2022)
1 more

1 mention without citation

Pseudomonas aeruginosa C-Terminal Processing Protease CtpA Assembles into a Hexameric Structure That Requires Activation by a Spiral-Shaped Lipoprotein-Binding Partner.
Hsu et al. (2022)