› 7rwa

Electron Microscopy
4.7Å resolution

AP2 bound to heparin and Tgn38 tyrosine cargo peptide

Released:
DOI: 10.2210/pdb7rwa/pdb
PDB entry 7rwa coloured by chain, front view.
PDB entry 7rwa coloured by chain, side view.
PDB entry 7rwa coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of an endocytic checkpoint that primes the AP2 clathrin adaptor for cargo internalization.
Partlow EA, Cannon KS, Hollopeter G, Baker RW
Nat Struct Mol Biol 29 339-347 (2022)
PMID: 35347313
Related structures: EMD-24712

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-148064 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-2 complex subunit alpha-2 Chains: A, a
Molecule details ›
Chains: A, a
Length: 630 amino acids
Theoretical weight: 70.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17427 (Residues: 1-271, 272-620; Coverage: 66%)
Gene names: Adtab, Ap2a2
Sequence domains: Adaptin N terminal region
AP-2 complex subunit beta Chains: B, b
Molecule details ›
Chains: B, b
Length: 591 amino acids
Theoretical weight: 66.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9DBG3 (Residues: 1-591; Coverage: 63%)
Gene names: Ap2b1, Clapb1
Sequence domains: Adaptin N terminal region
AP-2 complex subunit mu Chains: M, m
Molecule details ›
Chains: M, m
Length: 435 amino acids
Theoretical weight: 49.73 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84091 (Residues: 1-435; Coverage: 100%)
Gene names: Ap2m1, Clapm1
Sequence domains:
AP-2 complex subunit sigma Chains: S, s
Molecule details ›
Chains: S, s
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62743 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain
Trans-Golgi network integral membrane protein 2 Chains: Y, y
Molecule details ›
Chains: Y, y
Length: 15 amino acids
Theoretical weight: 1.81 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q62314 (Residues: 346-359; Coverage: 4%)
Gene names: Tgoln2, Ttgn2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.7Å
Relevant EMDB volumes: EMD-24712
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

SGIP1 in axons prevents internalization of desensitized CB1R and modifies its function.
Durydivka et al. (2023)
4 other articles

1 mention without citation

Structural basis of an endocytic checkpoint that primes the AP2 clathrin adaptor for cargo internalization.
Partlow et al. (2022)