› 7rx6

X-ray diffraction
1.8Å resolution

Structure of METTL3-METTL14(R298C) mutant methyltransferase complex

Released:
DOI: 10.2210/pdb7rx6/pdb
PDB entry 7rx6 coloured by chain, front view.
PDB entry 7rx6 coloured by chain, side view.
PDB entry 7rx6 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wang P, Nam Y

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183176 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N(6)-adenosine-methyltransferase catalytic subunit METTL3 Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86U44 (Residues: 357-580; Coverage: 39%)
Gene names: METTL3, MTA70
Sequence domains: MT-A70
N(6)-adenosine-methyltransferase non-catalytic subunit METTL14 Chain: B
Molecule details ›
Chain: B
Length: 349 amino acids
Theoretical weight: 39.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HCE5 (Residues: 111-456; Coverage: 76%)
Gene names: KIAA1627, METTL14
Sequence domains: MT-A70

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 99.743Å b: 99.743Å c: 118.378Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.219
Expression system: Escherichia coli

Quick links