› 7rxc

Electron Microscopy
3.2Å resolution

CryoEM structure of KDELR with Legobody

Released:
DOI: 10.2210/pdb7rxc/pdb
PDB entry 7rxc coloured by chain, front view.
PDB entry 7rxc coloured by chain, side view.
PDB entry 7rxc coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies).
Wu X, Rapoport TA
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 34620716
Related structures: EMD-24728

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-236999 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Fab_8D3_2 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 234 amino acids
Theoretical weight: 25.25 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Fab_8D3_2 light chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.1 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Nb_KR Chain: N
Immunoglobulin G-binding protein A; Immunoglobulin G-binding protein G Chain: B
ER lumen protein-retaining receptor 2 Chain: K

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand POV 4 x POV
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.2Å
Relevant EMDB volumes: EMD-24728
Expression systems:
  • Homo sapiens
  • Escherichia coli
  • Saccharomyces cerevisiae

Quick links

Citations

13 review citations

Protein Design: From the Aspect of Water Solubility and Stability.
Qing et al. (2022)
12 more