› 7s06

Electron Microscopy
3.3Å resolution

Cryo-EM structure of human GlcNAc-1-phosphotransferase A2B2 subcomplex

Released:
DOI: 10.2210/pdb7s06/pdb
PDB entry 7s06 coloured by chain, front view.
PDB entry 7s06 coloured by chain, side view.
PDB entry 7s06 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human GlcNAc-1-phosphotransferase αβ subunits reveals regulatory mechanism for lysosomal enzyme glycan phosphorylation.
Li H, Lee WS, Feng X, Bai L, Jennings BC, Liu L, Doray B, Canfield WM, Kornfeld S, Li H
Nat Struct Mol Biol 29 348-356 (2022)
PMID: 35332324
Related structures: EMD-24785

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-D-glucosamine + lysosomal-enzyme D-mannose = UMP + lysosomal-enzyme N-acetyl-D-glucosaminyl-phospho-D-mannose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
N-acetylglucosamine-1-phosphotransferase subunits alpha/beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 1179 amino acids
Theoretical weight: 134.79 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q3T906 (Residues: 44-1209; Coverage: 93%)
Gene names: GNPTA, GNPTAB, KIAA1208
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Ligand NAG 8 x NAG
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-24785
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

3'-sulfated LewisA/C: An oncofetal epitope associated with metaplastic and oncogenic plasticity of the gastrointestinal foregut.
Das et al. (2023)
1 more

1 mention without citation

Structure of the human GlcNAc-1-phosphotransferase αβ subunits reveals regulatory mechanism for lysosomal enzyme glycan phosphorylation.
Li et al. (2022)