› 7s2x

X-ray diffraction
2.85Å resolution

Structure of SalC, a gamma-lactam-beta-lactone bicyclase for salinosporamide biosynthesis

Released:
DOI: 10.2210/pdb7s2x/pdb
PDB entry 7s2x coloured by chain, front view.
PDB entry 7s2x coloured by chain, side view.
PDB entry 7s2x coloured by chain, top view.
Source organism: Salinispora tropica
Primary publication:
Enzymatic assembly of the salinosporamide γ-lactam-β-lactone anticancer warhead.
Bauman KD, Shende VV, Chen PY, Trivella DBB, Gulder TAM, Vellalath S, Romo D, Moore BS
OpenAccess logo Nat Chem Biol 18 538-546 (2022)
PMID: 35314816

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109171 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketosynthase family 3 (KS3) domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 597 amino acids
Theoretical weight: 64.88 KDa
Source organism: Salinispora tropica
Expression system: Streptomyces coelicolor
UniProt:
  • Canonical: B0L7F9 (Residues: 1-597; Coverage: 100%)
Gene name: salC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand K 4 x K
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 84.33Å b: 218.29Å c: 87.87Å
α: 90° β: 111.014° γ: 90°
R-values:
R R work R free
0.199 0.197 0.23
Expression system: Streptomyces coelicolor

Quick links

Citations

2 review citations

Noncanonical Functions of Enzyme Cofactors as Building Blocks in Natural Product Biosynthesis.
Barra et al. (2022)
1 more

1 mention without citation

Enzymatic assembly of the salinosporamide γ-lactam-β-lactone anticancer warhead.
Bauman et al. (2022)