› 7s37

Electron Microscopy
3.2Å resolution

Cas9:sgRNA (S. pyogenes) in the open-protein conformation

Released:
DOI: 10.2210/pdb7s37/pdb
PDB entry 7s37 coloured by chain, front view.
PDB entry 7s37 coloured by chain, side view.
PDB entry 7s37 coloured by chain, top view.
Source organisms:
Primary publication:
CRISPR-Cas9 bends and twists DNA to read its sequence.
Cofsky JC, Soczek KM, Knott GJ, Nogales E, Doudna JA
Nat Struct Mol Biol 29 395-402 (2022)
PMID: 35422516
Related structures: EMD-24818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189434 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: P
Molecule details ›
Chain: P
Length: 1371 amino acids
Theoretical weight: 158.97 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
Single-guide RNA Chain: R
Molecule details ›
Chain: R
Length: 102 nucleotides
Theoretical weight: 32.79 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-24818
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Structural biology of CRISPR-Cas immunity and genome editing enzymes.
Wang et al. (2022)
9 more

1 mention without citation

CRISPR-Cas9 bends and twists DNA to read its sequence.
Cofsky et al. (2022)